DNA Cloning: The
Basics
Restriction
Endonucleases and DNA Ligase Yield Recombinant
DNA
Cloning
Vectors Amplify Inserted DNA Segments
Isolating a Gene
from a Cellular Chromosome
Cloning a
Gene Often Requires a DNA Library
Hybridization
Identifies Specific Sequences in a DNA Library
BOX 28-1 A New
Weapon in Forensic Medicine
Specific
DNA Sequences Can Be Amplified
The Products of
Recombinant DNA Technology
Cloned
Genes Can Be Expressed
Cloned
Genes Can Be Altered
Yeast Is
an Important Eukaryotic Host for Recombinant DNA
Cloning
in Plants Is Aided by a Bacterial Parasite
Cloning
in Animal Cells Points the Way to Human Gene
Therapy
BOX 28-2 A Cure for
Genetic Diseases
Recombinant
DNA Technology Yields New Products and Choices
Summay
Further Reading
Problem
